Journal: Journal of visualized experiments : JoVE

Article Title: Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

doi: 10.3791/60996

Figure Lengend Snippet: Table of Materials

Article Snippet: Schematic of workflow of xenografting zebrafish larvae and performing drug screen, including imaging on a fluorescence microscope equipped imaging unit and automated sampler unit. table ft1 table-wrap mode="anchored" t5 Name of Material/Equipment Company Catalog Number Comments/Description 10× TBE Liquid Concentrate VWR 0658-5L 96-well plate, flat bottom CELLTREAT 229195 VAST is compatible with a variety of standard or deep well 24, 48, or 96 well plates Agarose Fisher Scientific BP160-500 Borosilicate Glass Capillary without Filament Sutter Instrument Company B100-50-10 Dexamethasone Enzo Life Sciences BML-EI126-0001 DMSO Sigma-Aldrich D2438-5X10ML E3 media N/A 5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, 0.33 mM MgSO4 Femtotips Microloader Tips Eppendorf 930001007 Fetal Bovine Serum (Premium Heat Inactivated) Atlanta Biologicals S11150H ImageJ FIJI N/A https://imagej.net/Fiji Iscove’s Modified Dulbecco’s Medium STEMCELL Technologies 36150 Large Particle (LP) Sampler Union Biometrica N/A http://www.unionbio.com/copas/features.aspx?id=8 Methotrexate Sigma-Aldrich A6770-10MG Mineral Oil Fisher Scientific BP26291 Phosphate Buffered Saline (1×) Caisson labs PBL06-6X500ML Stage Micrometer (400-Stage) Hausser Scientific 400-S Tricaine-S Pentair Aquatic TRS1 Trypan Blue Thermo Fisher {"type":"entrez-nucleotide","attrs":{"text":"T10282","term_id":"471631","term_text":"T10282"}} T10282 VAST Bioimager Union Biometrica N/A https://www.unionbio.com/vast/ Vincristine Sulfate Enzo Life Sciences BML-T117-0005 Vybrant DiI Stain Thermo Fisher V22885 Open in a separate window We have developed a streamlined workflow for xenografting primary patient leukemia cells into zebrafish and performing high-throughput drug screens with automated imaging and quantification, which can be applied to any other primary patient tumor cells or cancer cell line.

Techniques: Modification, Saline, Staining